Mps1(Mph1) Kinase phosphorylates Mad3 to Inhibit Cdc20Slp1 –APC/C and maintain spindle checkpoint arrests

ABSTRACT: The spindle checkpoint is a mitotic surveillance system which ensures equal segregation of sister chromatids. It delays anaphase onset by inhibiting the action of the E3 ubiquitin ligase known as the anaphase promoting complex or cyclosome (APC/C). Mads/BubR1 is a key component of the mitotic checkpoint complex (MCC) which binds and inhibits the APC/C early in mitosis. Mps1Mph1 kinase is critical for checkpoint signalling and MCC-APC/C inhibition, yet few substrates have been identified. Here we identify Mad3 as a substrate of fission yeast Mps1Mph1 kinase. We map and mutate phosphorylation sites in Mad3, producing mutants that are targeted to kinetochores and assembled into MCC, yet display reduced APC/C binding and are unable to maintain checkpoint arrests. We chow biochemically that Mad3 phospho-mimics are potent APC/C inhibitors in vitro, demonstrating that Mad3p modification can directly influence Cdc20Slp1-APC/C activity. This genetic dissection of APC/C inhibition demonstrates that Mps1Mph1 kinase-dependant modification of Mad3 and Mad2 act in a concerted manner to maintain spindle checkpoint arrests.

INSTRUMENT(S):

ORGANISM(S): Schizosaccharomyces Pombe

TISSUE(S): Cell Suspension Culture, Vegetative Cell (sensu Fungi)

SUBMITTER: Heather Barker  

LAB HEAD: Kevin Hardwick

PROVIDER: PXD003806 | Pride | 2016-03-31

REPOSITORIES: Pride