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Human AML patient samples, Method Optimization, LC-MSMS

ABSTRACT: We evaluated different in-solution and filter-aided sample preparation (FASP) proteomic workflows, and different enrichment strategies of phosphorylated peptides on acute myeloid leukemia (AML) patient samples. We also studied the effect of liquid nitrogen storage on the proteome and phosphoproteome of four AML patients.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Non-terminally Differentiated Cell

DISEASE(S): Acute Myeloid Leukemia

SUBMITTER: Elise Aasebo

LAB HEAD: Frode Steingrimsen Berven

PROVIDER: PXD003822 | Pride | 2016-04-26

REPOSITORIES: Pride

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Freezing effects on the acute myeloid leukemia cell proteome and phosphoproteome revealed using optimal quantitative workflows.

Aasebø Elise E Mjaavatten Olav O Vaudel Marc M Farag Yehia Y Selheim Frode F Berven Frode F Bruserud Øystein Ø Hernandez-Valladares Maria M

Journal of proteomics 20160420

<h4>Unlabelled</h4>MS-based proteomic studies aiming for the discovery of acute myeloid leukemia (AML) biomarkers require sample processing that can assure an optimal proteome coverage and identification of PTMs. We evaluated different in-solution and filter-aided sample preparation (FASP) proteomic workflows and different enrichment strategies of phosphorylated peptides. The FASP protocols in the label-free and SILAC (stable isotope labelling with amino acids in cell culture) approaches were se ...[more]

PMID: 27107777

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