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Tube-gel digestion: a fast and reproducible sample preparation protocol for label-free quantitative proteomics of complex biological samples

ABSTRACT: Comparison of tube-gel protocol to stacking gel and liquid digestion for label-free extracted ion-current quantification of complex biological samples Dataset with known fold-changes (UPS1 spiked in yeast background at 25-10-5-2.5-1-0.5 fmol)

INSTRUMENT(S): Q Exactive

ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast)

SUBMITTER: Leslie MULLER

LAB HEAD: Sarah CIANFERANI

PROVIDER: PXD003841 | Pride | 2016-10-18

REPOSITORIES: Pride

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Benchmarking sample preparation/digestion protocols reveals tube-gel being a fast and repeatable method for quantitative proteomics.

Muller Leslie L Fornecker Luc L Van Dorsselaer Alain A Cianférani Sarah S Carapito Christine C

Proteomics 20161201 23

Sample preparation, typically by in-solution or in-gel approaches, has a strong influence on the accuracy and robustness of quantitative proteomics workflows. The major benefit of in-gel procedures is their compatibility with detergents (such as SDS) for protein solubilization. However, SDS-PAGE is a time-consuming approach. Tube-gel (TG) preparation circumvents this drawback as it involves directly trapping the sample in a polyacrylamide gel matrix without electrophoresis. We report here the fi ...[more]

PMID: 27749015

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