Identification of Proteins Sensitive to Thermal Stress in Human HEK293 cells

ABSTRACT: Heat-shock is an acute insult to the mammalian proteome. The sudden elevation in temperature has far-reaching effects on protein metabolism, leads to a rapid inhibition of most protein synthesis, and the induction of protein chaperones. Using heat-shock in human HEK293 cells, in conjunction with detergent extraction and sedimentation followed by LC-MS/MS proteomic approaches, we identify proteins that lose solubility upon heat-shock. We assume that these are metastable in protein homeostasis.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture, Early Embryonic Cell

DISEASE(S): Disease Free

SUBMITTER: Guilian Xu  

LAB HEAD: David R. Borchelt

PROVIDER: PXD003888 | Pride | 2016-04-11

REPOSITORIES: Pride