Ontology highlight
ABSTRACT:
INSTRUMENT(S): QSTAR
ORGANISM(S): Homo Sapiens (human)
TISSUE(S): Cell Culture, Early Embryonic Cell
DISEASE(S): Disease Free
SUBMITTER: Guilian Xu
LAB HEAD: David R. Borchelt
PROVIDER: PXD003888 | Pride | 2016-04-11
REPOSITORIES: Pride
Xu Guilian G Pattamatta Amrutha A Hildago Ryan R Pace Michael C MC Brown Hilda H Borchelt David R DR
Journal of cell science 20160329 9
The capacity of the cell to produce, fold and degrade proteins relies on components of the proteostasis network. Multiple types of insults can impose a burden on this network, causing protein misfolding. Using thermal stress, a classic example of acute proteostatic stress, we demonstrate that ∼5-10% of the soluble cytosolic and nuclear proteome in human HEK293 cells is vulnerable to misfolding when proteostatic function is overwhelmed. Inhibiting new protein synthesis for 30 min prior to heat-sh ...[more]