AP-MS analysis of m7GTP-binding proteins in Murine norovirus infected BV-2 cells

ABSTRACT: SILAC-labelling analysis of murine BV-2 cells infected at high multiplicity of infection (10 TCID50/cell) and harvested at 4h or 9h post-infection. Lysates were then suject to m7GTP-sepharose affinity purification to enrich for translation initiation factors. As norovirus translation uses a VPg protein covalently linked to the 5' of its RNAs for initiation factor recruitment it was hypothesised that norovirus infection could modify initiation factor complexes. A paired dataset investigates the effects of MNV infection on total protein levels in infected cells (PXD004015).

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Cell Culture, Microglial Cell

DISEASE(S): Norovirus Gastroenteritis

SUBMITTER: Edward Emmott  

LAB HEAD: Ian Goodfellow

PROVIDER: PXD004019 | Pride | 2016-07-07

REPOSITORIES: Pride