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Glycoproteomes of naive and LPS stimulated human monocytes and THP-1 cells

ABSTRACT: CD14+ Monocytes from healthy volunteers were purified by MACS (negative selection) and FACSorting and either left untreated or stimulated for 24h and 48h with LPS. THP-1 cells were stimulated for 4h, 24h and 48h with LPS. Glycoproteins were captured with hydrazide chemistry and tryptic and PNGase F-released peptide fractions analyzed by MS/MS. Quantitative assessment revealed differential glycoprotein expression in activated/LPS-tolerized monocytes and naïve monocytes and THP-1 cells.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Monocyte, Blood

SUBMITTER: Mario Müller

LAB HEAD: Hortense Slevogt

PROVIDER: PXD004034 | Pride | 2017-04-24

REPOSITORIES: Pride

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Global analysis of glycoproteins identifies markers of endotoxin tolerant monocytes and GPR84 as a modulator of TNFα expression.

Müller Mario M MM Lehmann Roland R Klassert Tilman E TE Reifenstein Stella S Conrad Theresia T Moore Christoph C Kuhn Anna A Behnert Andrea A Guthke Reinhard R Driesch Dominik D Slevogt Hortense H

Scientific reports 20170412 1

Exposure of human monocytes to lipopolysaccharide (LPS) induces a temporary insensitivity to subsequent LPS challenges, a cellular state called endotoxin tolerance. In this study, we investigated the LPS-induced global glycoprotein expression changes of tolerant human monocytes and THP-1 cells to identify markers and glycoprotein targets capable to modulate the immunosuppressive state. Using hydrazide chemistry and LC-MS/MS analysis, we analyzed glycoprotein expression changes during a 48 h LPS ...[more]

PMID: 28404994

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