Dataset Information

WPip (Buckeye) Ovarian Proteome

ABSTRACT: A protein pilot dataset detecting Wolbachia proteins from protein extracted from dissected infected Culex pipiens mosquito ovaries. The experiment was based of an iTRAQ experiment comparing infected and uninfected ovarian tissues and has been usefull in characterizing the wPip (Buckeye) ovarian proteome.

OTHER RELATED OMICS DATASETS IN: PRJNA213627PRJNA319204

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Wolbachia Pipientis Subsp. Culex Pipiens (strain Wpip)

TISSUE(S): Ovary

SUBMITTER: John Beckmann

LAB HEAD: John Beckmann

PROVIDER: PXD004047 | Pride | 2017-03-06

REPOSITORIES: Pride

ACCESS DATA

Publications

Prophage WO genes recapitulate and enhance Wolbachia-induced cytoplasmic incompatibility.

LePage Daniel P DP Metcalf Jason A JA Bordenstein Sarah R SR On Jungmin J Perlmutter Jessamyn I JI Shropshire J Dylan JD Layton Emily M EM Funkhouser-Jones Lisa J LJ Beckmann John F JF Bordenstein Seth R SR

Nature 20170227 7644

The genus Wolbachia is an archetype of maternally inherited intracellular bacteria that infect the germline of numerous invertebrate species worldwide. They can selfishly alter arthropod sex ratios and reproductive strategies to increase the proportion of the infected matriline in the population. The most common reproductive manipulation is cytoplasmic incompatibility, which results in embryonic lethality in crosses between infected males and uninfected females. Females infected with the same Wo ...[more]

PMID: 28241146

Similar Datasets

Project description:Using Solexa/Illumina's digital gene expression (DGE) system, a tag-based transcriptome sequencing method, we investigated the kinetic transcriptional profile of gene expression in macrophages infected with Brucella melitensis strain 16M. A key aspect of Brucella virulence is their ability to proliferate within professional and nonprofessional phagocytic host cells, thereby successfully bypassing the bactericidal effects of phagocytes. Their virulence and chronic infections are thought to be due to their ability to avoid the killing mechanisms within host cells. Defining the interaction between a host cell and Brucella is crucial to understanding the infectious process. Most researchers have studied the pathogens, but the host plays a very important role during infections. To date, relatively few host factors have been shown important in Brucella infections. However, little is known about the host networks that mediate infection. The objective of the study is to analyze the genes and cellular components related to the innate immunity response to determine the mechanisms through which Brucella avoids the host innate immunity. A total of 3576 and 3962 genes that are differentially expressed between 0 and 4 h and between 0 and 24 h were identified. The identified genes are related to immune processes, signal transduction, inflammation, apoptosis, cell membrane, transcriptional regulation, and intracellular trafficking. Our data have added to the current understanding of different host gene expressions during different infection phases by Brucella spp. The RAW264.7 cells were seeded into 24-well plates at 5-105 cells/well, incubated in 5% CO2 at 37 M-BM-0C for 24 h, and then infected with Brucella at a multiplicity of infection of 200. To synchronize the infection, the infected plates were centrifuged at 200 ug for 5 min at room temperature and then incubated at 37 M-BM-0C for 20 minutes. The infected cell monolayers were washed three times with PBS, overlaid with 0.5 ml of DMEM containing 100 mg/ml of ampicillin and 50 mg/ml of kanamycin at 37 M-BM-0C for 0, 4, and 24 h. At the end of the incubation period, the culture medium was removed and centrifuged at 1500 rpm for 15 min. The cell pellet and adherent cells were resuspended in TRIzol. Total RNA were extracted from the cells at 0, 4 and 24 h post infection.

Dataset Information

WPip (Buckeye) Ovarian Proteome

Publications

Prophage WO genes recapitulate and enhance Wolbachia-induced cytoplasmic incompatibility.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure