SILAC analysis of Δhmt1 yeast - Knockout of the Hmt1p arginine methyltransferase in Saccharomyces cerevisiae leads to the dysregulation of phosphate-associated genes and processes

ABSTRACT: In this study, the proteome-level effects of the deletion of the gene encoding Hmt1p, the predominant yeast arginine methyltransferase, were measured using SILAC (stable isotope labeling by amino acids in cell culture), to clarify if the abundance levels of any proteins were disrupted by systemic loss of arginine methylation. Analysis revealed that numerous proteins were found to be differentially abundant between wild-type yeast and Δhmt1, and functional analysis revealed perturbation of phosphate signalling and repression of the PHO pathway.

OTHER RELATED OMICS DATASETS IN: GSE99869

INSTRUMENT(S):

ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast)

SUBMITTER: Daniel Yagoub  

LAB HEAD: Marc Roland Wilkins

PROVIDER: PXD004054 | Pride | 2018-11-21

REPOSITORIES: Pride