Proteomics,Multiomics

Dataset Information

0

SILAC analysis of Δhmt1 yeast - Knockout of the Hmt1p arginine methyltransferase in Saccharomyces cerevisiae leads to the dysregulation of phosphate-associated genes and processes

ABSTRACT: In this study, the proteome-level effects of the deletion of the gene encoding Hmt1p, the predominant yeast arginine methyltransferase, were measured using SILAC (stable isotope labeling by amino acids in cell culture), to clarify if the abundance levels of any proteins were disrupted by systemic loss of arginine methylation. Analysis revealed that numerous proteins were found to be differentially abundant between wild-type yeast and Δhmt1, and functional analysis revealed perturbation of phosphate signalling and repression of the PHO pathway.

OTHER RELATED OMICS DATASETS IN: GSE99869

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast)

SUBMITTER: Daniel Yagoub  

LAB HEAD: Marc Roland Wilkins

PROVIDER: PXD004054 | Pride | 2018-11-21

REPOSITORIES: Pride

Json Xml
altmetric image

Publications

Knockout of the Hmt1p Arginine Methyltransferase in <i>Saccharomyces cerevisiae</i> Leads to the Dysregulation of Phosphate-associated Genes and Processes.

Chia Samantha Z SZ   Lai Yu-Wen YW   Yagoub Daniel D   Lev Sophie S   Hamey Joshua J JJ   Pang Chi Nam Ignatius CNI   Desmarini Desmarini D   Chen Zhiliang Z   Djordjevic Julianne T JT   Erce Melissa A MA   Hart-Smith Gene G   Wilkins Marc R MR  

Molecular & cellular proteomics : MCP 20180911 12

Hmt1p is the predominant arginine methyltransferase in <i>Saccharomyces cerevisiae</i> Its substrate proteins are involved in transcription, transcriptional regulation, nucleocytoplasmic transport and RNA splicing. Hmt1p-catalyzed methylation can also modulate protein-protein interactions. Hmt1p is conserved from unicellular eukaryotes through to mammals where its ortholog, PRMT1, is lethal upon knockout. In yeast, however, the effect of knockout on the transcriptome and proteome has not been de  ...[more]

Similar Datasets

Proteomics Investigating the upstream signaling of Imh1 phosphorylation under ER stress in yeast
2022-07-21 | PXD031563 | Pride
Proteomics Yeast glutamine tRNA synthetase doxycycline shut-off
2020-02-12 | PXD017140 | Pride
Transcriptomics Transcriptome regulation in response to respiratory inhibition with antimycin A in: (a) wild type fission yeast grown in minimal media (EMM) (b) minimal media supplemented with arginine or in (c) strain with deletion of ssp1 gene.
2020-03-30 | E-MTAB-8805 | biostudies-arrayexpress
Transcriptomics Transcription profiling of normal and tumorigenic Rattus norvegicus cell lines grown under normal and arginine deprivation conditions
2007-10-08 | E-GEOD-2275 | biostudies-arrayexpress
Proteomics Nanodiscs-based proteomics to identify lipid-interacting proteins in yeast
2021-12-30 | PXD027992 | Pride
Proteomics Identification of Golgin Imh1 phosphorylation residues under ER stress in yeast
2022-07-21 | PXD031562 | Pride
Transcriptomics Saccharomyces cerevisiae mutant strain with R1158 strain background: Fold change after doxycycline treatment
2014-03-26 | E-GEOD-56186 | biostudies-arrayexpress
Proteomics MS2-Deisotoper: A tool for deisotoping high-resolution MS/MS spectra in normal and heavy isotope-labelled samples
2019-08-05 | PXD010587 | Pride
Transcriptomics ArgR1 and AhrC Mediate Arginine-Dependent Regulation of Arginine Acquisition- and Virulence Genes in the Human Pathogen Streptococcus pneumoniae
2011-11-02 | E-GEOD-33043 | biostudies-arrayexpress
Proteomics Effect of acadesine and overexpression of the karyopherin Kap123 on nuclear abundance of yeast proteins
2018-11-23 | PXD007779 | Pride