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Comparative iTRAQ analysis of proteomic expression in human sinoatrial node and working cardiomyocytes

ABSTRACT: The goal of this study was to assess the changes in protein expression in the human sinoatrial node (SAN) compared with working cardiomyocytes in order to identify specific SAN protein signatures. Four pair of samples (the SAN and working cardiomyocytes) were obtained post-mortem from four healthy human donors.

INSTRUMENT(S): LTQ

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Heart

SUBMITTER: Maciej Suski

LAB HEAD: Paweł Wołkow

PROVIDER: PXD004109 | Pride | 2018-03-05

REPOSITORIES: Pride

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Comparative iTRAQ analysis of protein abundance in the human sinoatrial node and working cardiomyocytes.

Klimek-Piotrowska Wiesława W Krawczyk-Ożóg Agata A Suski Maciej M Kapusta Przemysław P Wołkow Paweł P PP Hołda Mateusz K MK

Journal of anatomy 20180227 6

Our objective was to assess the changes in protein abundance in the human sinoatrial node (SAN) compared with working cardiomyocytes to identify SAN-specific protein signatures. Four pairs of samples (the SAN and working cardiomyocytes) were obtained postmortem from four human donors with no evidence of cardiovascular disease. We performed protein identification and quantitation using two-dimensional chromatography-tandem mass spectrometry with isobaric peptide labeling (iTRAQ). We identified 45 ...[more]

PMID: 29484645

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