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Proteomics needs better chromatography

ABSTRACT: Examination of relationship between chromatographic peak capacity and MS/MS scan rate.

INSTRUMENT(S): Orbitrap Fusion ETD

ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast)

SUBMITTER: Evgenia Shishkova

LAB HEAD: Joshua J Coon

PROVIDER: PXD004126 | Pride | 2016-12-13

REPOSITORIES: Pride

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Now, More Than Ever, Proteomics Needs Better Chromatography.

Shishkova Evgenia E Hebert Alexander S AS Coon Joshua J JJ

Cell systems 20161001 4

From plant research to biomedicine, proteome analysis plays a critical role in many areas of biological inquiry. Steady improvement in mass spectrometer (MS) technology has transformed the speed and depth of proteome analysis. Proteomes of simple organisms can now be sequenced to near completion in just over an hour. Comparable coverage of mammalian proteomes, however, still requires hours or even days of analysis. Here we ask why current technology fails to achieve comprehensive and rapid analy ...[more]

PMID: 27788355

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