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Personalized proteome profiles of healthy and tumor human colon organoids reveal both individual diversity and basic features of colorectal cancer.

ABSTRACT: We performed quantitative mass spectrometry based proteomic analysis and comparative transcriptomics analysis of 7 individual patient-derived human colorectal tumor and healthy organoids, the latter acquired from adjacent healthy tissue.

INSTRUMENT(S): LTQ Orbitrap Elite, Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Colon

DISEASE(S): Colon Cancer

SUBMITTER: Alba Cristobal

LAB HEAD: Albert J. R. Heck

PROVIDER: PXD004149 | Pride | 2017-01-16

REPOSITORIES: Pride

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Personalized Proteome Profiles of Healthy and Tumor Human Colon Organoids Reveal Both Individual Diversity and Basic Features of Colorectal Cancer.

Cristobal Alba A van den Toorn Henk W P HWP van de Wetering Marc M Clevers Hans H Heck Albert J R AJR Mohammed Shabaz S

Cell reports 20170101 1

Diseases at the molecular level are complex and patient dependent, necessitating development of strategies that enable precision treatment to optimize clinical outcomes. Organoid technology has recently been shown to have the potential to recapitulate the in vivo characteristics of the original individual's tissue in a three-dimensional in vitro culture system. Here, we present a quantitative mass-spectrometry-based proteomic analysis and a comparative transcriptomic analysis of human colorectal ...[more]

PMID: 28052255

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