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Proteomic Time-Course Investigation into the Effects of Exposure of Mycobacterium smegmatis to Sub-Lethal Dose of Rifampicin

ABSTRACT: Drug susceptible Mycobacterium smegmatis (Mc2-155) was exposed to a sub-MIC concentration of antimycobacterial drug Rifampicin (treated) or DMSO (control). Cells were harvested at 30, 255 and 300 minutes post-exposure and the proteome at each time analysed using LC-MS/MS on a Thermo Q-Exactive.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Mycobacterium Smegmatis (strain Atcc 700084 / Mc(2)155)

DISEASE(S): Tuberculosis

SUBMITTER: Alexander Giddey

LAB HEAD: Jonathan M Blackburn

PROVIDER: PXD004197 | Pride | 2017-03-15

REPOSITORIES: Pride

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A temporal proteome dynamics study reveals the molecular basis of induced phenotypic resistance in Mycobacterium smegmatis at sub-lethal rifampicin concentrations.

Giddey Alexander D AD de Kock Elise E Nakedi Kehilwe C KC Garnett Shaun S Nel Andrew J M AJ Soares Nelson C NC Blackburn Jonathan M JM

Scientific reports 20170306

In the last 40 years only one new antitubercular drug has been approved, whilst resistance to current drugs, including rifampicin, is spreading. Here, we used the model organism Mycobacterium smegmatis to study mechanisms of phenotypic mycobacterial resistance, employing quantitative mass spectrometry-based proteomics to investigate the temporal effects of sub-lethal concentrations of rifampicin on the mycobacterial proteome at time-points corresponding to early response, onset of bacteriostasis ...[more]

PMID: 28262820

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