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Identification of potential human PML interacting proteins in Nicotiana benthamiana

ABSTRACT: In spite of the crucial tasks fulfilled by the subnuclear structures known as PML nuclear bodies (PML-NBs) in mammalian cells, the presence of PML-NBs in plant cells could not be shown yet. Interestingly, the key organizer of PML-NBs, human promyelocytic leukemia protein (PML), is not targeted to structures resembling PML-NBs when expressed heterologously in plants, but localizes to interchromosomal areas associated with the nucleolus. In order to identify plant proteins interacting with human PML, a GFP-fusion protein was ectopically expressed in Nicotiana benthamiana using Agrobacterium tumefaciens mediated transformation. The bait protein as well as potential interactors were immunoprecipitated by use of magnetic GFP-Trap beads (Chromotek). Precipitated proteins were analyzed by Nano-LC-MS/MS.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Nicotiana Benthamiana

TISSUE(S): Plant Cell, Leaf

DISEASE(S): Disease Free

SUBMITTER: Christian Lamm

LAB HEAD: Uwe Sonnewald

PROVIDER: PXD004254 | Pride | 2016-10-10

REPOSITORIES: Pride

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