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Circadian profiling of the Arabidopsis proteome using 2D-DIGE

ABSTRACT: Clock-generated biological rhythms provide an adaptive advantage to an organism which results in increased fitness and survival. To better elucidate the plant response to the circadian system, we surveyed protein oscillations in Arabidopsis seedlings under constant light. Using large-scale two-dimensional differential in-gel electrophoresis (2D-DIGE) the abundance of more than one thousand proteins spots was reproducibly resolved, quantified and profiled across a circadian time series. A comparison between phenol-extracted samples and Rubisco-depleted extracts identified 70 and 39 rhythmically-expressed proteins, respectively.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

TISSUE(S): Plant Cell, Seedling

SUBMITTER: Hirofumi Nakagami

LAB HEAD: Hirofumi Nakagami

PROVIDER: PXD004276 | Pride | 2016-06-29

REPOSITORIES: Pride

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Circadian Profiling of the Arabidopsis Proteome Using 2D-DIGE.

Choudhary Mani K MK Nomura Yuko Y Shi Hua H Nakagami Hirofumi H Somers David E DE

Frontiers in plant science 20160712

Clock-generated biological rhythms provide an adaptive advantage to an organism, resulting in increased fitness and survival. To better elucidate the plant response to the circadian system, we surveyed protein oscillations in Arabidopsis seedlings under constant light. Using large-scale two-dimensional difference in gel electrophoresis (2D-DIGE) the abundance of more than 1000 proteins spots was reproducibly resolved quantified and profiled across a circadian time series. A comparison between ph ...[more]

PMID: 27462335

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