Quantitative Secretome Analysis of Activated Jurkat Cells

ABSTRACT: A quantitative label-free secretome analysis protocol using a click chemistry-based approach for the enrichment of secreted glycoproteins was adapted for and applied to a T cell model. There, Jurkat cells were activated via PMA/ionomycin and the dynamic modulation of the T cell secretome was investigated and compared to the dynamic modulation of the T cell proteome of the same model.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): T Cell, Jurkat Cell

DISEASE(S): T-cell Leukemia

SUBMITTER: Thilo Bracht  

LAB HEAD: Barbara Sitek

PROVIDER: PXD004280 | Pride | 2016-10-10

REPOSITORIES: Pride