Dataset Information

Mouse Liver LC-MSMS - System-wide quantitative proteomics of the metabolic syndrome in mice: genotypic and dietary effects

ABSTRACT: Advances in mass spectrometry have made the quantitative measurement of proteins across multiple samples a reality, allowing for the study of complex biological systems such as the metabolic syndrome. Although deregulation of lipid metabolism and increased hepatic storage of triacylglycerides are known to play a part in the onset of the metabolic syndrome, its molecular basis and dependency on dietary and genotypic factors are poorly characterized. Here, we used a rich experimental design with two different mouse strains, dietary and metabolic perturbations to generate a compendium of quantitative proteome data, using three mass spectrometry strategies. The data recapitulates known properties of the metabolic system and indicate differential molecular adaptation of the two mouse strains to perturbations, contributing to a better understanding of the metabolic syndrome. We show that high-quality, high-throughput proteomic datasets provide an unbiased broad overview of complex systems upon perturbation.

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Hepatocyte, Liver

DISEASE(S): Type 2 Diabetes Mellitus

SUBMITTER: Eduard Sabidó

LAB HEAD: Ruedi Aebersold

PROVIDER: PXD004284 | Pride | 2016-12-22

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	esabido_B1003_226.c.mzXML	Mzxml
	esabido_B1003_227.c.mzXML	Mzxml
	esabido_B1003_228.c.mzXML	Mzxml
	esabido_B1003_230.c.mzXML	Mzxml
	esabido_B1003_231.c.mzXML	Mzxml

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Publications

System-Wide Quantitative Proteomics of the Metabolic Syndrome in Mice: Genotypic and Dietary Effects.

Terfve Camille C Sabidó Eduard E Wu Yibo Y Gonçalves Emanuel E Choi Meena M Vaga Stefania S Vitek Olga O Saez-Rodriguez Julio J Aebersold Ruedi R

Journal of proteome research 20170110 2

Advances in mass spectrometry have made the quantitative measurement of proteins across multiple samples a reality, allowing for the study of complex biological systems such as the metabolic syndrome. Although the deregulation of lipid metabolism and increased hepatic storage of triacylglycerides are known to play a part in the onset of the metabolic syndrome, its molecular basis and dependency on dietary and genotypic factors are poorly characterized. Here, we used an experimental design with t ...[more]

PMID: 27936760

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