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Cysteine-targeted proteomics analysis

ABSTRACT: The protein cysteine residue is one of the amino acids most susceptible to oxidative modifications, frequently caused by oxidative stress. Several applications have enabled cysteine-targeted proteomics analysis with simultaneous detection and quantitation. In this study, we employed a quantitative approach using a set of iodoacetyl-based cysteine reactive isobaric tags (iodoTMT) and evaluated the transient cellular oxidation ratio of free and reversibly modified cysteine thiols under DTT and hydrogen peroxide (H2O2) treatments.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Kidney

SUBMITTER: Kazutaka Araki

LAB HEAD: Kazuhiko Fukui

PROVIDER: PXD004303 | Pride | 2016-07-18

REPOSITORIES: Pride

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Redox Sensitivities of Global Cellular Cysteine Residues under Reductive and Oxidative Stress.

Araki Kazutaka K Kusano Hidewo H Sasaki Naoyuki N Tanaka Riko R Hatta Tomohisa T Fukui Kazuhiko K Natsume Tohru T

Journal of proteome research 20160715 8

The protein cysteine residue is one of the amino acids most susceptible to oxidative modifications, frequently caused by oxidative stress. Several applications have enabled cysteine-targeted proteomics analysis with simultaneous detection and quantitation. In this study, we employed a quantitative approach using a set of iodoacetyl-based cysteine reactive isobaric tags (iodoTMT) and evaluated the transient cellular oxidation ratio of free and reversibly modified cysteine thiols under DTT and hyd ...[more]

PMID: 27350002

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