Analysis of mixed-oxysterol treatment on the THP-1 macrophage

ABSTRACT: THP-1 monocytes were differentiated, via PMA treatment, to macrophages. THP-1 macrophages were then treated with a mixed-oxysterol treatment (7-ketocholesterol and 7beta-hydroxycholesterol) for 24 hours. The mixed-oxysterol treatment was mixed to a ratio that represents the cytotoxic oxysterol content of human atherosclerotic plaque. Control and treated macrophages were analysed using both 2-DE with peptide mass-fingerprinting and tandem-mass spectrometry.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture, Macrophage

DISEASE(S): Arteriosclerosis

SUBMITTER: Liam Ward  

LAB HEAD: Xi-Ming Yuan

PROVIDER: PXD004304 | Pride | 2017-03-15

REPOSITORIES: Pride