Proteomics

Dataset Information

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Analysis of mixed-oxysterol treatment on the THP-1 macrophage


ABSTRACT: THP-1 monocytes were differentiated, via PMA treatment, to macrophages. THP-1 macrophages were then treated with a mixed-oxysterol treatment (7-ketocholesterol and 7beta-hydroxycholesterol) for 24 hours. The mixed-oxysterol treatment was mixed to a ratio that represents the cytotoxic oxysterol content of human atherosclerotic plaque. Control and treated macrophages were analysed using both 2-DE with peptide mass-fingerprinting and tandem-mass spectrometry.

INSTRUMENT(S): LTQ Orbitrap Velos, Voyager-DE PRO

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture, Macrophage

DISEASE(S): Arteriosclerosis

SUBMITTER: Liam Ward  

LAB HEAD: Xi-Ming Yuan

PROVIDER: PXD004304 | Pride | 2017-03-15

REPOSITORIES: Pride

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Publications

Exposure to atheroma-relevant 7-oxysterols causes proteomic alterations in cell death, cellular longevity, and lipid metabolism in THP-1 macrophages.

Ward Liam J LJ   Ljunggren Stefan A SA   Karlsson Helen H   Li Wei W   Yuan Xi-Ming XM  

PloS one 20170328 3


The 7-oxysterols are recognised as strong enhancers of inflammatory processes in foamy macrophages. Atheroma-relevant 7-oxysterol mixtures induce a mixed type of cell death in macrophages, and trigger cellular oxidative stress responses, which mimic oxidative exposures observed in atherosclerotic lesions. However, the macrophage proteome has not previously been determined in the 7-oxysterol treated cell model. The aim of the present study was to determine the specific effects of an atheroma-rele  ...[more]

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