Dataset Information


Quantitative proteomics of liver from PCB 153 exposed Atlantic cod

ABSTRACT: In order to investigate the underlying mechanisms of PCB 153 mediated toxicity to Atlantic cod (Gadus morhua), we analyzed the liver proteome of fish exposed to various doses of PCB 153 (0, 0.5, 2 and 8mg/kg body weight) for two weeks and examined the effects on expression of liver proteins using quantitative proteomics. Label-free mass spectrometry enabled quantification of 1272 proteins, and 78 were differentially regulated between PCB 153 treated samples and controls. Two proteins downregulated due to PCB 153 treatment, Glutathione S-transferase theta 1 (GSTT1) and sulfotransferase family protein 1 (ST2B1), were verified using selected reaction monitoring (SRM). Supported by bioinformatics analyses, we concluded that PCB 153 perturbs lipid metabolism in the Atlantic cod liver and that increased levels of lipogenic enzymes indicate increased synthesis of fatty acids and triglycerides.

INSTRUMENT(S): LTQ Orbitrap Velos, QTRAP 5500

ORGANISM(S): Gadus morhua  

TISSUE(S): Liver

DISEASE(S): Not Available

SUBMITTER: Odd Andre Karlsen  

LAB HEAD: Odd Andre Karlsen

PROVIDER: PXD004362 | Pride | 2017-01-17


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Quantitative proteomics analysis reveals perturbation of lipid metabolic pathways in the liver of Atlantic cod (Gadus morhua) treated with PCB 153.

Yadetie Fekadu F   Oveland Eystein E   Døskeland Anne A   Berven Frode F   Goksøyr Anders A   Karlsen Odd André OA  

Aquatic toxicology (Amsterdam, Netherlands) 20170130

PCB 153 is one of the most abundant PCB congeners detected in biological samples. It is a persistent compound that is still present in the environment despite the ban on production and use of PCBs in the late 1970s. It has strong tendencies to bioaccumulate and biomagnify in biota, and studies have suggested that it is an endocrine and metabolic disruptor. In order to study mechanisms of toxicity, we exposed Atlantic cod (Gadus morhua) to various doses of PCB 153 (0, 0.5, 2 and 8mg/kg body weigh  ...[more]

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