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O-fucosylated glycoproteins of Toxoplasma gondii

ABSTRACT: The fucose-binding Aleuria aurantia lectin (AAL) labels punctiform structures in proximity to the nuclei of Toxoplasma gondii tachyzoites. We have utilized AAL-lectin affinity purification in conjunction with LC MS/MS to identify a set of proteins modified with fucose on serines and threonines

INSTRUMENT(S): Agilent instrument model, Q Exactive

ORGANISM(S): Toxoplasma Gondii

SUBMITTER: John Haserick

LAB HEAD: Catherine E. Costello

PROVIDER: PXD004426 | Pride | 2016-10-11

REPOSITORIES: Pride

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O-fucosylated glycoproteins form assemblies in close proximity to the nuclear pore complexes of Toxoplasma gondii.

Bandini Giulia G Haserick John R JR Motari Edwin E Ouologuem Dinkorma T DT Lourido Sebastian S Roos David S DS Costello Catherine E CE Robbins Phillips W PW Samuelson John J

Proceedings of the National Academy of Sciences of the United States of America 20160923 41

Toxoplasma gondii is an intracellular parasite that causes disseminated infections in fetuses and immunocompromised individuals. Although gene regulation is important for parasite differentiation and pathogenesis, little is known about protein organization in the nucleus. Here we show that the fucose-binding Aleuria aurantia lectin (AAL) binds to numerous punctate structures in the nuclei of tachyzoites, bradyzoites, and sporozoites but not oocysts. AAL also binds to Hammondia and Neospora nucle ...[more]

PMID: 27663739

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