Dataset Information
Proteomics of the phytopathogen Dickeya dadantii
Ontology highlight
ABSTRACT:
INSTRUMENT(S): Q Exactive
ORGANISM(S): Dickeya Dadantii Subsp. Dadantii
TISSUE(S): Cell Culture
SUBMITTER: 
Sophie Ayciriex
LAB HEAD: Jérôme Lemoine
PROVIDER: PXD004438 | Pride | 2017-01-02
REPOSITORIES: Pride
Publications
Scout-MRM: Multiplexed Targeted Mass Spectrometry-Based Assay without Retention Time Scheduling Exemplified by Dickeya dadantii Proteomic Analysis during Plant Infection.
Analytical chemistry 20170109 3
Targeted mass spectrometry of a surrogate peptide panel is a powerful method to study the dynamics of protein networks, but chromatographic time scheduling remains a major limitation for dissemination and implementation of robust and large multiplexed assays. We unveil a Multiple Reaction Monitoring method (Scout-MRM) where the use of spiked scout peptides triggers complex transition lists, regardless of the retention time of targeted surrogate peptides. The interest of Scout-MRM method regardin ...[more]
