Proteomics

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Comparison of Protein Extraction Methods in Tendon Mass Spectrometry Analysis


ABSTRACT: Tendons play a fundamental role in the musculoskeletal system and locomotion by transferring forces generated from muscles to the skeleton. Tendon injuries can occur due to sports related incidents, as a result of trauma to overuse or during disease or ageing. Using proteomic techniques tendon protein profiles could be defined under different conditions. The aim of this study was to optimise tendon sample preparation for mass spectrometry analysis, which will be valuable for future tendon studies.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Equus Caballus (horse)

TISSUE(S): Tendon

SUBMITTER: Yalda Ashraf Kharaz  

LAB HEAD: Mandy J Peffers

PROVIDER: PXD004453 | Pride | 2018-07-05

REPOSITORIES: Pride

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Publications

Comparison between chaotropic and detergent-based sample preparation workflow in tendon for mass spectrometry analysis.

Ashraf Kharaz Yalda Y   Zamboulis Danae D   Sanders Karen K   Comerford Eithne E   Clegg Peter P   Peffers Mandy M  

Proteomics 20170622 13-14


Exploring the tendon proteome is a challenging but important task for understanding the mechanisms of physiological/pathological processes during ageing and disease and for the development of new treatments. Several extraction methods have been utilised for tendon mass spectrometry, however different extraction methods have not been simultaneously compared. In the present study we compared protein extraction in tendon with two chaotropic agents, guanidine hydrochloride (GnHCl) and urea, a deterg  ...[more]

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