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Proteasome degradation of synthetic ubiquitinated globin

ABSTRACT: Analysis of the peptides resulting from proteasome-mediated degradation of K48-linked tetra ubiquitin conjugated to alpha globin

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Oded Kleifeld

LAB HEAD: Michael Glickman

PROVIDER: PXD004456 | Pride | 2021-08-24

REPOSITORIES: Pride

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Synthetic Uncleavable Ubiquitinated Proteins Dissect Proteasome Deubiquitination and Degradation, and Highlight Distinctive Fate of Tetraubiquitin.

Singh Sumeet K SK Sahu Indrajit I Mali Sachitanand M SM Hemantha Hosahalli P HP Kleifeld Oded O Glickman Michael H MH Brik Ashraf A

Journal of the American Chemical Society 20161206 49

Various hypotheses have been proposed regarding how chain length, linkage type, position on substrate, and susceptibility to deubiquitinases (DUBs) affect processing of different substrates by proteasome. Here we report a new strategy for the chemical synthesis of ubiquitinated proteins to generate a set of well-defined conjugates bearing an oxime bond between the chain and the substrate. We confirmed that this isopeptide replacement is resistant to DUBs and to shaving by proteasome. Analyzing p ...[more]

PMID: 27960333

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