Dataset Information

Alterations in kernel proteome after infection with Fusarium culmorum in two triticale cultivars with contrasting resistance to Fusarium head blight

ABSTRACT: Triticale was used as a model to recognize new components of molecular mechanism of resistance to Fusarium head blight (FHB) in cereals. Fusarium-damaged kernels of two lines distinct in levels of resistance to FHB were applied into a proteome profiling using two-dimensional gel electrophoresis (2-DE) to create protein maps and mass spectrometry to identify the proteins differentially accumulated between the analyzed lines. The 2-DE analysis indicated a total of 23 spots with clear differences in a protein content between the more resistant and more susceptible triticale lines after infection with F. culmorum. A majority of the proteins were involved in a cell carbohydrate metabolism, stressing the importance of this protein group in a plant response to Fusarium infection. The increased accumulation levels of different isoforms of plant beta-amylase were observed for a more susceptible triticale line after inoculation. The more resistant line was characterized by a higher abundance of alpha-amylase inhibitor CM2 subunit.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Secale Cereale X Triticum Turgidum Subsp. Durum

TISSUE(S): Leaf

SUBMITTER: Agata Malinowska

LAB HEAD: Michal Dadlez

PROVIDER: PXD004464 | Pride | 2018-10-27

REPOSITORIES: Pride

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Publications

Alterations in Kernel Proteome after Infection with Fusarium culmorum in Two Triticale Cultivars with Contrasting Resistance to Fusarium Head Blight.

Perlikowski Dawid D Wiśniewska Halina H Kaczmarek Joanna J Góral Tomasz T Ochodzki Piotr P Kwiatek Michał M Majka Maciej M Augustyniak Adam A Kosmala Arkadiusz A

Frontiers in plant science 20160817

Highlight: The level of pathogen alpha-amylase and plant beta-amylase activities could be components of plant-pathogen interaction associated with the resistance of triticale to Fusarium head blight. Triticale was used here as a model to recognize new components of molecular mechanism of resistance to Fusarium head blight (FHB) in cereals. Fusarium-damaged kernels (FDK) of two lines distinct in levels of resistance to FHB were applied into a proteome profiling using two-dimensional gel electroph ...[more]

PMID: 27582751

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Dataset Information

Alterations in kernel proteome after infection with Fusarium culmorum in two triticale cultivars with contrasting resistance to Fusarium head blight

Publications

Alterations in Kernel Proteome after Infection with Fusarium culmorum in Two Triticale Cultivars with Contrasting Resistance to Fusarium Head Blight.

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