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LC-MS/MS of dithiothreitol-extracted proteins from the insect fungal pathogen Beauveria bassiana conidia

ABSTRACT: Proteins were extracted using 2 mM DTT from Beauveria bassiana conidia were identified and quantified using Label-free quantitative mass spectrometry.

INSTRUMENT(S):

ORGANISM(S): Beauveria Bassiana Cgmcc 7.34

TISSUE(S): Fungal Asexual Spore, Conidium

SUBMITTER: zhi Yang

LAB HEAD: Yongjun Zhang

PROVIDER: PXD004474 | Pride | 2022-03-02

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	2-CO.group	Other
	CO.group	Other
	beauveriabassiana-CO-1-2.mgf	Mgf
	beauveriabassiana-CO-1-2.wiff	Wiff
	beauveriabassiana-CO-1.mgf	Mgf

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LC-MS/MS of dithiothreitol-extracted proteins from the insect fungal pathogen Beauveria bassiana conidia

Project description:Proteins were extracted using 2 mM DTT from Beauveria bassiana conidia were identified and quantified using Label-free quantitative mass spectrometry.

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LC-MS/MS of dithiothreitol-extracted proteins from the insect fungal pathogen Beauveria bassiana blastospores

Project description:Proteins were extracted using 2 mM DTT from Beauveria bassiana blastospores were identified and quantified using Label-free quantitative mass spectrometry.

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LC-MS/MS of dithiothreitol-extracted proteins from the insect fungal pathogen Beauveria bassiana hyphal bodies (in vivo blastospores)

Project description:Proteins were extracted using 2 mM DTT from Beauveria bassiana hyphal bodies (in vivo blastospores) were identified and quantified using Label-free quantitative mass spectrometry.

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The insufficient activation of RIG-I-like signaling pathway in IBRS-2 cells contributes to highly efficient replication of porcine picornaviruses

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The insufficient activation of RIG-I-like signaling pathway in IBRS-2 cells contributes to highly efficient replication of porcine picornaviruses

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A proteomics analysis of adventitious root formation after leaf removal in lotus (Nelumbo nucifera Gaertn.)

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Pancreatic cancer cells treated with anlotinib

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Project description:Pretreated human follicular fluid was prepared for targeted metabolomics experiments. For this purpose, 12-point standard calibration curves in the respective range with reference material were measured, and the limit of detection (LOD) and limit of quantification (LOQ) for each neurotransmitter under investigation were calculated. LC-MS/MS was conducted by an ultra-performance liquid chromatography method using a Waters ACQUITY UPLC BEH C18 column (2.1 mm×100 mm, 1.7 µm, Waters, USA). The Waters acquisition UPLC was coupled to an API 4000 triple quadrupole mass spectrometer (AB SCIEX, USA). The mobile phase A buffer was 10% methanol in water (containing 0.1% formic acid), and the mobile phase B buffer was 50% methanol in water (containing 0.1% formic acid). The LC gradient elution was at a flow rate of 0.3 mL/min at 0-8.0 min and 0.4 ml/min at 8.0-17.5 min, then 10%-30% B at 0-6.5 min; 30%-100% B at 6.5-7 min; 100% B at 7-14min; and 100%-10% B at 14-17.5min. The injection volume was 5 µl and the column temperature was set to 40 °C. Twenty-three metabolites (Ach: Acetylcholine chloride; Gln: Glutamine; Glu: Glutamate; His: L-Histidine; NE: norepinephrine; Tyr:Tyrosine; Trp:Tryptophan; Kyn: Kynurenine; GABA: 4-Aminobutyric acid;HisA:Histamine; PA:Picolinic acid;TyrA:Tyramine;DA:Hydroxytyramine hydrochloride; TrpA:Tryptamine;5-HT:Serotonin hydrochloride;E:Adrenaline hydrochloride;KynA:Kynurenic acid;5-HIAA:5-Hydroxyindole-3-acetic acid;DOPA:Levodopa;XA:Xanthurenic acid;VWA:Vanillymandelic Acid; 5-HTTP:5-Hydroxytryptophan;MT:Melatonine)were simultaneously reported in LC-MS/MS multiple reaction monitoring (MRM) mode. Data analysis was performed with Analyst 1.6 software.

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