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Sheep fecal microbiota - Diversity and functions of the sheep faecal microbiota: a multi-omic characterization

ABSTRACT: Characterization of the fecal metaproteome of dairy sheep

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Microbiota

TISSUE(S): Gastrointestinal Tract

SUBMITTER: Alessandro Tanca

LAB HEAD: Sergio Uzzau

PROVIDER: PXD004524 | Pride | 2017-02-22

REPOSITORIES: Pride

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Diversity and functions of the sheep faecal microbiota: a multi-omic characterization.

Tanca Alessandro A Fraumene Cristina C Manghina Valeria V Palomba Antonio A Abbondio Marcello M Deligios Massimo M Pagnozzi Daniela D Addis Maria Filippa MF Uzzau Sergio S

Microbial biotechnology 20170206 3

Little is currently known on the microbial populations colonizing the sheep large intestine, despite their expected key role in host metabolism, physiology and immunity. This study reports the first characterization of the sheep faecal microbiota composition and functions, obtained through the application of a multi-omic strategy. An optimized protocol was first devised for DNA extraction and amplification from sheep stool samples. Then, 16S rDNA sequencing, shotgun metagenomics and shotgun meta ...[more]

PMID: 28165194

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