Repo-Man/PP1 regulates heterochromatin formation in interphase

ABSTRACT: GST:Repo-Man(C-terminus) or GST:alone were expressed as in Vagnarelli et al. 2011. Before elution, beads were first incubated with nucleosomes extracted from HeLa. HeLa cells were lysed and nucleosomes were digested with MNase (20min, 37C). Nucleosomes were firstly incubated with GST alone for 2hrs. This cleared lysate was then incubated with GST:Repo-Man and GST alone. Beads were then eluted and mix with 3x Laemli Buffer. Histone bands were sent to Mass Spec.

OTHER RELATED OMICS DATASETS IN: PRJNA327850

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Paola Vagnarelli  

LAB HEAD: Paola Vagnarelli

PROVIDER: PXD004613 | Pride | 2016-12-07

REPOSITORIES: Pride