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Cancer proteome and metabolite changes linked to SHMT2

ABSTRACT: To screen for proximate and interacting proteins of SHMT2 in HeLa cells, the BioID method was employed, which consists of biotinylation of proteins in the vicinity of FlagBirAR118G –SHMT2 followed by streptavidin-based affinity capture and mass spectrometry identification. Experimental procedures were carried out essentially as described previously.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Permanent Cell Line Cell

SUBMITTER: Jiefei Tong

LAB HEAD: Michael F Moran

PROVIDER: PXD004629 | Pride | 2020-08-12

REPOSITORIES: Pride

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Proteomics and metabolomics reveal changes in cancer cells after alternation of SHMT2 expressions

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