Jurkat 2D-LC-MS/MS - Enzyme Kinetics for Complex System Enables Accurate Determination of Specificity Constants of Numerous Substrates in a Mixture by Proteomics Platform

ABSTRACT: We found the ratio of substrate depletion is independent of other coexisted substrates under specific condition with the application of self-developed iteration approach. The further established simplified model was applied to determine the catalytic efficiencies of peptide substrates of a protease in a complex reaction system.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): T Cell, Cell Culture

DISEASE(S): Leukemia

SUBMITTER: Zhenzhen Deng  

LAB HEAD: Mingliang Ye

PROVIDER: PXD004665 | Pride | 2017-02-08

REPOSITORIES: Pride