Proteomics

Dataset Information

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Switchgrass drought-induced leaf proteomics analysis


ABSTRACT: Switchgrass (Panicum virgatum) is a perennial crop producing deep roots thus highly tolerant to soil water deficit conditions. However, seedling establishment in field is very susceptible to prolonged and periodic drought stress. In this study, a “sandwich” system simulating a gradual water deletion process was developed. Switchgrass seedlings were subjected to a 20-day gradual drought treatment process when soil water tension was increased to 0.05 MPa (moderate drought stress) and leaf physiological properties had expressed significant alteration. Drought-induced changes in leaf proteomes were identified using the relative and absolute quantitation (iTRAQ) labeling method followed by nano-scale liquid chromatography mass spectrometry (nano-LC-MS/MS) analysis. Additionally, total leaf proteins were processed using a combinatorial library of peptide ligands to enrich for lower abundance proteins. Both total proteins and those enriched samples were analyzed to increase the coverage of the quantitative proteomics analysis. A total of 7,006 leaf proteins were identified, and 257 (4% of the leaf proteome) expressed a significant difference (P < 0.05, fold change < 0.6 or > 1.7) from the non-treated control to drought-treated conditions. Results from this study, in addition to revealing molecular responses to drought stress, provide a large number of proteins (candidate genes) that can be employed to improve switchgrass seedling growth and establishment under soil drought condition.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Panicum Virgatum (switchgrass)

TISSUE(S): Leaf

SUBMITTER: Ted Thannhauser  

LAB HEAD: Ted Thannhauser

PROVIDER: PXD004675 | Pride | 2016-08-09

REPOSITORIES: Pride

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Publications

Drought-Induced Leaf Proteome Changes in Switchgrass Seedlings.

Ye Zhujia Z   Sangireddy Sasikiran S   Okekeogbu Ikenna I   Zhou Suping S   Yu Chih-Li CL   Hui Dafeng D   Howe Kevin J KJ   Fish Tara T   Thannhauser Theodore W TW  

International journal of molecular sciences 20160802 8


Switchgrass (Panicum virgatum) is a perennial crop producing deep roots and thus highly tolerant to soil water deficit conditions. However, seedling establishment in the field is very susceptible to prolonged and periodic drought stress. In this study, a "sandwich" system simulating a gradual water deletion process was developed. Switchgrass seedlings were subjected to a 20-day gradual drought treatment process when soil water tension was increased to 0.05 MPa (moderate drought stress) and leaf  ...[more]

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