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Proteomic Quantification of Phosphorylation in Mouse HT22 Cells

ABSTRACT: The aim of this project is to use an integrated approach involving SILAC labeling, HPLC fractionation, Phospho(STY) antibody affinity enrichment, LC-MS/MS to quantify the dynamic changes of the whole phosphorylome in the control and experimental samples.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Cell Culture, Neuroblast

DISEASE(S): Neuroblastoma

SUBMITTER: zhang hong gang

LAB HEAD: hong gang zhang

PROVIDER: PXD004687 | Pride | 2018-10-27

REPOSITORIES: Pride

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Quantitative analyses of the global proteome and phosphoproteome reveal the different impacts of propofol and dexmedetomidine on HT22 cells.

Zhang Honggang H Ye Juan J Shi Zhaomei Z Bu Chen C Bao Fangping F

Scientific reports 20170418

Propofol and dexmedetomidine are both commonly used anaesthetics. Although they employ two different mechanisms to induce anaesthesia, both compounds influence the hippocampus and the HT22 cell line. HT22 cells are broadly used in neurobiological research. In this study, we assessed the effects of propofol and dexmedetomidine on signalling in HT22 cells. Using the SILAC (stable isotope labelling with amino acids in cell culture) labelling technique, IMAC (immobilized metal affinity chromatograph ...[more]

PMID: 28418008

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