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Transcription complexes in the embryo

ABSTRACT: In this study, we characterized the composition of TFIID and SAGA complexes in the mouse embryo and analyzed the effect of the depletion of a shared subunit TAF10 during development

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Embryo

SUBMITTER: Mathilde Joint

LAB HEAD: Stéphane VINCENT

PROVIDER: PXD004688 | Pride | 2017-09-22

REPOSITORIES: Pride

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The TAF10-containing TFIID and SAGA transcriptional complexes are dispensable for early somitogenesis in the mouse embryo.

Bardot Paul P Vincent Stéphane D SD Fournier Marjorie M Hubaud Alexis A Joint Mathilde M Tora László L Pourquié Olivier O

Development (Cambridge, England) 20170911 20

During development, tightly regulated gene expression programs control cell fate and patterning. A key regulatory step in eukaryotic transcription is the assembly of the pre-initiation complex (PIC) at promoters. PIC assembly has mainly been studied <i>in vitro</i>, and little is known about its composition during development. <i>In vitro</i> data suggest that TFIID is the general transcription factor that nucleates PIC formation at promoters. Here we show that TAF10, a subunit of TFIID and of t ...[more]

PMID: 28893950

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