Proteomics

Dataset Information

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Comprehensive identification of proteins expressed by human oligodendroglia cell line

ABSTRACT: Oligodendrocytes are a type of neuroglia that have provide trophic support and insulation to axons in central nervous system. A proper differentiation of oligodendrocyte precursor cells is crucial for central nervous system myelination and development. Such maturation proccess can be induced in vitro by addition of 12-myristate 13-acetate (PMA) jointly to serum deprivation in oligodendrocyte precursor cell cultures. In the present work, we established a reference data set using 2D LC fractionation and ion-mobility enhanced data-independent proteomic analyses of proteins expressed by treated- and nontreated oligodendrocytes. The final dataset beyond all fractions comprised 223,531 ion peptides corresponding to 10,614 proteins groups. Our results can help futher studies using MO3.13 cells as a tool of investigation not only to oligodendrocyte maturation, also to diseases that have oligodendrocytes as key players.

INSTRUMENT(S): Synapt MS

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Juliana Cassoli  

LAB HEAD: Daniel Martins de Souza

PROVIDER: PXD004696 | Pride | 2017-09-27

REPOSITORIES: Pride

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Publications

Ion Mobility-Enhanced Data-Independent Acquisitions Enable a Deep Proteomic Landscape of Oligodendrocytes.

S Cassoli Juliana J   Brandão-Teles Caroline C   G Santana Aline A   H M F Souza Gustavo G   Martins-de-Souza Daniel D  

Proteomics 20171016 21

Oligodendrocytes are a type of neuroglia that provide trophic support and insulation to axons in the central nervous system. The genesis and maturation of oligodendrocytes are essential processes for myelination and the course of CNS development. Using ion mobility-enhanced, data-independent acquisitions and 2D-nanoUPLC fractionation operating at nanoscale flow rates, we established a comprehensive data set of proteins expressed by the human oligodendroglia cell line MO3.13. The final dataset in  ...[more]

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