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Urinary proteome of a healthy human cohort aged 70 to 82

ABSTRACT: 12 urine samples from 6 individuals (2 time points each) enrolled in a study on healthy aging were collected. Samples were frozen at -80⁰C prior to analysis. Samples were thawed, pH neutralized, separated into soluble urine (SU) and urinary pellet (UP) fractions, and subjected to filter aided sample preparation (FASP). FASP-processed samples were subjected to LC-MS/MS analysis on a Q Exactive mass spec.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Urine

SUBMITTER: Yanbao Yu

LAB HEAD: Yanbao Yu

PROVIDER: PXD004713 | Pride | 2017-05-03

REPOSITORIES: Pride

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Publications

Comprehensive Metaproteomic Analyses of Urine in the Presence and Absence of Neutrophil-Associated Inflammation in the Urinary Tract.

Yu Yanbao Y Sikorski Patricia P Smith Madeline M Bowman-Gholston Cynthia C Cacciabeve Nicolas N Nelson Karen E KE Pieper Rembert R

Theranostics 20170101 2

Inflammation in the urinary tract results in a urinary proteome characterized by a high dynamic range of protein concentrations and high variability in protein content. This proteome encompasses plasma proteins not resorbed by renal tubular uptake, renal secretion products, proteins of immune cells and erythrocytes derived from trans-urothelial migration and vascular leakage, respectively, and exfoliating urothelial and squamous epithelial cells. We examined how such proteins partition into solu ...[more]

PMID: 28042331

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