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A systematic proteogenomic approach to deciphering of the missing proteins: the example of newly uncovered NHERF1 function associated with human reproductive disorder

ABSTRACT: We propose and demonstrate a systematic approach designed to combine stringent criteria for claims of missing protein (MP) identification with extensive annotation as critical components of the discovery and functional validation of MPs.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Placenta

SUBMITTER: Jin-Young Cho

LAB HEAD: Young-Ki Paik

PROVIDER: PXD004723 | Pride | 2018-01-05

REPOSITORIES: Pride

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Systematic Proteogenomic Approach To Exploring a Novel Function for NHERF1 in Human Reproductive Disorder: Lessons for Exploring Missing Proteins.

Na Keun K Shin Heon H Cho Jin-Young JY Jung Sang Hee SH Lim Jaeseung J Lim Jong-Sun JS Kim Eun Ah EA Kim Hye Sun HS Kang Ah Reum AR Kim Ji Hye JH Shin Jeong Min JM Jeong Seul-Ki SK Kim Chae-Yeon CY Park Jun Young JY Chung Hyung-Min HM Omenn Gilbert S GS Hancock William S WS Paik Young-Ki YK

Journal of proteome research 20171101 12

One of the major goals of the Chromosome-Centric Human Proteome Project (C-HPP) is to fill the knowledge gaps between human genomic information and the corresponding proteomic information. These gaps are due to "missing" proteins (MPs)-predicted proteins with insufficient evidence from mass spectrometry (MS), biochemical, structural, or antibody analyses-that currently account for 2579 of the 19587 predicted human proteins (neXtProt, 2017-01). We address some of the lessons learned from the inco ...[more]

PMID: 28960081

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