2D-LC/MS/MS analysis of Escherichia coli during exposure to abrupt downshifts in temperature and water activity

ABSTRACT: 2D-LC/MS/MS analysis was used to examine time-dependent changes in proteome of E. coli O157:H7 strain Sakai upon abrupt downshifts from 35°C aw 0.993 to 14°C aw 0.967. Bacterial cells were harvested before abrupt downshifts in both temperature and aw (i.e. control), or at 0 (i.e. immediately after the shift), 60, 250, 1605, 4,070, 5,700, 9,900 or 18,565 min after the shifts.

INSTRUMENT(S):

ORGANISM(S): Escherichia Coli

SUBMITTER: Chawalit Kocharunchitt  

LAB HEAD: Chawalit Kocharunchitt

PROVIDER: PXD004803 | Pride | 2016-09-29

REPOSITORIES: Pride