Proteomics

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Spectral histopathology supported laser capture microdissection and quantitative proteome analysis of diffuse malignant mesothelioma

ABSTRACT: FTIR based spectral histopathology was used for label-free classification of tumor tissue sections. The regions of interest were identified and annotated with high spatial resolution. The spatial information was used for automated laser capture microdissection of relevant tissue areas. Subsequently, the collected tissue samples were analyzed using quantitative proteomics. This novel approach for precise sample collection in clinical studies was applied to differentiate epitheloid and sarcomatoid subtypes of diffuse malignant mesothelioma.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Lung, Meso-epithelium, Pleura

DISEASE(S): Malignant Mesothelioma

SUBMITTER: Thilo Bracht  

LAB HEAD: Barbara Sitek

PROVIDER: PXD004818 | Pride | 2017-03-31

REPOSITORIES: Pride

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Publications

Spatial and molecular resolution of diffuse malignant mesothelioma heterogeneity by integrating label-free FTIR imaging, laser capture microdissection and proteomics.

Großerueschkamp Frederik F   Bracht Thilo T   Diehl Hanna C HC   Kuepper Claus C   Ahrens Maike M   Kallenbach-Thieltges Angela A   Mosig Axel A   Eisenacher Martin M   Marcus Katrin K   Behrens Thomas T   Brüning Thomas T   Theegarten Dirk D   Sitek Barbara B   Gerwert Klaus K  

Scientific reports 20170330

Diffuse malignant mesothelioma (DMM) is a heterogeneous malignant neoplasia manifesting with three subtypes: epithelioid, sarcomatoid and biphasic. DMM exhibit a high degree of spatial heterogeneity that complicates a thorough understanding of the underlying different molecular processes in each subtype. We present a novel approach to spatially resolve the heterogeneity of a tumour in a label-free manner by integrating FTIR imaging and laser capture microdissection (LCM). Subsequent proteome ana  ...[more]

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