Dataset Information

Proteomic analysis provides insights into the molecular bases of hydrogen gas-induced cadmium resistance in Medicago sativa

ABSTRACT: Recently, molecular hydrogen (H2) has emerged as a bio-regulator both in animals and plants. Normally, functions of endogenous generated H2 could be mimicked by exogenously applied hydrogen-rich water (HRW) or hydrogen-rich saline (particularly in animals). Although alfalfa seedlings showed more cadmium (Cd) resistance after the administration with HRW, corresponding molecular mechanism is still elusive. To address this gap, iTRAQ-based quantitative proteomics was used. In our experimental conditions, out of 238 significant differential proteins identified in the Cd- and HRW/Cd-treated samples, 60 were unique to Cd-stressed alone samples, 107 were unique to HRW plus Cd treatment samples, and 71 were shared between two groups. Furthermore, 58 proteins from the 238 proteins significantly responded in Cd- and HRW/Cd-treatment samples were selected for further bioinformatics analysis. Interestingly, results indicated that they were classified into six categories: oxidation-reduction process, carbon metabolism, citrate cycle, cysteine and methionine metabolism, metal ion homeostasis, and sulfur compound metabolic process. In addition, the protein expression patterns were consistent with the results of increased non-protein thiols abundant, as well as iron and zinc content, in seedling roots. These suggest that HRW alleviates Cd toxicity mainly by decreasing oxidative damage, enhancing sulfur compound metabolic process, and maintaining nutrient element homeostasis.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Medicago Sativa (alfalfa)

TISSUE(S): Plant Cell, Root

SUBMITTER: Chen Dai

LAB HEAD: Weiti Cui and Chen Dai

PROVIDER: PXD004820 | Pride | 2016-12-24

REPOSITORIES: Pride

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Proteomic analysis provides insights into the molecular bases of hydrogen gas-induced cadmium resistance in Medicago sativa.

Dai Chen C Cui Weiti W Pan Jincheng J Xie Yanjie Y Wang Jin J Shen Wenbiao W

Journal of proteomics 20161028

Recently, molecular hydrogen (H<sub>2</sub>) has emerged as a bio-regulator both in animals and plants. Normally, functions of endogenous generated H<sub>2</sub> could be mimicked by exogenously applied hydrogen-rich water (HRW) or hydrogen-rich saline (particularly in animals). Although alfalfa seedlings showed more cadmium (Cd) resistance after the administration with HRW, corresponding molecular mechanism is still elusive. To address this gap, iTRAQ-based quantitative proteomics was used. The ...[more]

PMID: 27989938

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Project description:Essential metals such as iron are required for healthy plant growth. Fe is an important cofactor and catalytic element in many biological processes. Fe and other metals can also be toxic when present in excess. Therefore plants have mechanisms of metal homeostasis which involve coordination of metal ion transporters for uptake, translocation and compartmentalisation. The NAS genes are supposed to play an important role in Fe homeostasis. They are coding for enzymes called nicotianaminesynthase (NAS), which synthesize nicotianamine (NA) by a one-step condensation reaction of three molecules S-adenosyl-methionine. NA acts as a chelator for Fe, Cu, Ni and Zn and might be involved in the transport and allocation of Fe throughout the plant. We generated quadruple T-DNA insertion mutant nas plants to investigate NA function as described in Klatte et al., 2009, Plant Physiol. The nas4x-1 plants show an interveinal leaf chlorosis when turning from vegetative to reproductive stage, which intensifies when growing under Fe deficiency conditions. nas4x-1 plants have strongly reduced NA contents and show an elevated Fe deficiency response in roots. By performing microarray experiments we want to reveal global changes on transcriptional level in roots and leaves of nas4x-1 mutant compared to wild type plants grown under Fe supply and Fe deficiency conditions, respectively. The loss of NAS genes has a strong impact on the regulation of other metal homeostasis genes and allows to draw conclusions about nicotianamine function in metal homeostasis of A.thaliana. For this study, four-week old nas4x-1 mutant and wild type plants were exposed for 7 days to plant medium with and without Fe supply. These conditions have been established previously and have resulted in a reproducibly strong interveinal leaf chlorosis of nas4x-1 plants compared to wild type, especially upon Fe deficiency conditions. The experiment was repeated three times in consecutive weeks to obtain three independent biological repetitions. Rosette leaves and roots of five week-old plants were harvested, RNA was isolated and microarray hybridization was performed. 24 Total samples were analyzed. We generated the following pairwise comparisons: WT + Fe vs. – Fe, nas4x-1 + Fe vs. – Fe, + Fe WT vs. + Fe nas4x-1, - Fe WT vs. - Fe nas4x-1, roots and leaves

Dataset Information

Proteomic analysis provides insights into the molecular bases of hydrogen gas-induced cadmium resistance in Medicago sativa

Publications

Proteomic analysis provides insights into the molecular bases of hydrogen gas-induced cadmium resistance in Medicago sativa.

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