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76 human liver tissue proteomes by PCT-SWATH

ABSTRACT: Rapid proteotyping of 38 human liver tissues in duplicate using pressure-cycling technology (PCT) and SWATH mass spectrometry

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Hepatocyte, Liver

DISEASE(S): Hepatocellular Carcinoma

SUBMITTER: Tiannan Guo

LAB HEAD: Ruedi Aebersold

PROVIDER: PXD004873 | Pride | 2018-10-31

REPOSITORIES: Pride

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Identification of Protein Abundance Changes in Hepatocellular Carcinoma Tissues Using PCT-SWATH.

Zhu Yi Y Zhu Jiang J Lu Cong C Zhang Qiushi Q Xie Wei W Sun Ping P Dong Xiaochuan X Yue Liang L Sun Yaoting Y Yi Xiao X Zhu Tiansheng T Ruan Guan G Aebersold Ruedi R Huang Shi'ang S Guo Tiannan T

Proteomics. Clinical applications 20181119 1

<h4>Purpose</h4>To rapidly identify protein abundance changes in biopsy-level fresh-frozen hepatocellular carcinoma (HCC).<h4>Experimental design</h4>The pressure-cycling technology (PCT) is applied and sequential window acquisition of all theoretical mass spectra (SWATH-MS) workflow is optimized to analyze 38 biopsy-level tissue samples from 19 HCC patients. Each proteome is analyzed with 45 min LC gradient. MCM7 is validated using immunohistochemistry (IHC).<h4>Results</h4>A total of 11 787 pr ...[more]

PMID: 30365225

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