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The bulk cell density as decisive parameter and simple method for mesendodermal patterning of human pluripotent stem cells

ABSTRACT: We showed that the relation of pluripotent stem cell count to culture volume influences cardiomyogenic differentiation by affecting the concentration of secreted proteins that function as paracrine factors

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Stem Cell

SUBMITTER: Falk Büttner

LAB HEAD: Rita Gerardy-Schahn

PROVIDER: PXD004874 | Pride | 2018-10-17

REPOSITORIES: Pride

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Bulk cell density and Wnt/TGFbeta signalling regulate mesendodermal patterning of human pluripotent stem cells.

Kempf Henning H Olmer Ruth R Haase Alexandra A Franke Annika A Bolesani Emiliano E Schwanke Kristin K Robles-Diaz Diana D Coffee Michelle M Göhring Gudrun G Dräger Gerald G Pötz Oliver O Joos Thomas T Martinez-Hackert Erik E Haverich Axel A Buettner Falk F R FFR Martin Ulrich U Zweigerdt Robert R

Nature communications 20161209

In vitro differentiation of human pluripotent stem cells (hPSCs) recapitulates early aspects of human embryogenesis, but the underlying processes are poorly understood and controlled. Here we show that modulating the bulk cell density (BCD: cell number per culture volume) deterministically alters anteroposterior patterning of primitive streak (PS)-like priming. The BCD in conjunction with the chemical WNT pathway activator CHIR99021 results in distinct paracrine microenvironments codifying hPSCs ...[more]

PMID: 27934856

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Modulation of the bulk cell density as decisive parameter and simple method for mesendodermal patterning of human pluripotent stem cells

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