Analysis of the psychrophilic fungus Mrakia psychrophila using shotgun proteomics

ABSTRACT: Comprehensive and quantitative information of the psychrophile proteome is an important source for understanding of the cold adaptation mechanism under low growth temperature. Mrakia psychrophila, a obligate fungus, was selected to quantitatively evaluate its protein abundance changes in response to three different temperatures. With optimized procedures of isobaric tags for relative and absolute quantitation quantitative proteomics(iTRAQ), high quality of the quantitative information of the peptides identified were acquired. In total, 1673 unique proteins were identified and defined 129 proteins as the temperature-dependent proteins. Function analysis revealed that energy metabolism is important for temperature adaptaion.

INSTRUMENT(S):

ORGANISM(S): Mrakia Psychrophila

TISSUE(S): Fungal Cell

SUBMITTER: Yao Su  

LAB HEAD: Xingzhong Liu

PROVIDER: PXD004881 | Pride | 2016-12-07

REPOSITORIES: Pride