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Analysis of the psychrophilic fungus Mrakia psychrophila using shotgun proteomics

ABSTRACT: Comprehensive and quantitative information of the psychrophile proteome is an important source for understanding of the cold adaptation mechanism under low growth temperature. Mrakia psychrophila, a obligate fungus, was selected to quantitatively evaluate its protein abundance changes in response to three different temperatures. With optimized procedures of isobaric tags for relative and absolute quantitation quantitative proteomics(iTRAQ), high quality of the quantitative information of the peptides identified were acquired. In total, 1673 unique proteins were identified and defined 129 proteins as the temperature-dependent proteins. Function analysis revealed that energy metabolism is important for temperature adaptaion.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Mrakia Psychrophila

TISSUE(S): Fungal Cell

SUBMITTER: Yao Su

LAB HEAD: Xingzhong Liu

PROVIDER: PXD004881 | Pride | 2016-12-07

REPOSITORIES: Pride

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Genomic, Transcriptomic, and Proteomic Analysis Provide Insights Into the Cold Adaptation Mechanism of the Obligate Psychrophilic Fungus Mrakia psychrophila.

Su Yao Y Jiang Xianzhi X Wu Wenping W Wang Manman M Hamid M Imran MI Xiang Meichun M Liu Xingzhong X

G3 (Bethesda, Md.) 20161108 11

Mrakia psychrophila is an obligate psychrophilic fungus. The cold adaptation mechanism of psychrophilic fungi remains unknown. Comparative genomics analysis indicated that M. psychrophila had a specific codon usage preference, especially for codons of Gly and Arg and its major facilitator superfamily (MFS) transporter gene family was expanded. Transcriptomic analysis revealed that genes involved in ribosome and energy metabolism were upregulated at 4°, while genes involved in unfol ...[more]

PMID: 27633791

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