Dataset Information
Ethylene glycol improves electrospray ionization
Ontology highlight
ABSTRACT:
INSTRUMENT(S): LTQ Orbitrap, LTQ Orbitrap Velos
ORGANISM(S): Homo Sapiens (human)
TISSUE(S): Cell Suspension Culture
SUBMITTER: 
Peng Yu
LAB HEAD: Bernhard Kuster
PROVIDER: PXD004919 | Pride | 2017-07-14
REPOSITORIES: Pride
Publications
Ethylene glycol improves electrospray ionization efficiency in bottom-up proteomics.
Analytical and bioanalytical chemistry 20161020 4
Liquid chromatography coupled online to nano-electrospray ionization (nESI) tandem mass spectrometry is the analytical workhorse in the field of proteome research. Dimethyl sulfoxide (DMSO) was recently shown to improve nESI efficiency by a factor of three to ten thus improving the sensitivity and coverage of proteomic experiments. However, relatively few investigations into which solvent additives promote nESI response have been performed at a proteomic scale. Here, we systematically evaluated ...[more]
