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Human intracranial tumors LC-MS/MS

ABSTRACT: We found the reality that each Coomassie blue-stained 2D gel spot contained lots of protein species in analysis of in tissue proteome.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Brain

DISEASE(S): Brain Cancer

SUBMITTER: Xianquan Zhan

LAB HEAD: Xianquan Zhan

PROVIDER: PXD004930 | Pride | 2017-12-11

REPOSITORIES: Pride

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How many proteins can be identified in a 2DE gel spot within an analysis of a complex human cancer tissue proteome?

Zhan Xianquan X Yang Haiyan H Peng Fang F Li Jianglin J Mu Yun Y Long Ying Y Cheng Tingting T Huang Yuda Y Li Zhao Z Lu Miaolong M Li Na N Li Maoyu M Liu Jianping J Jungblut Peter R PR

Electrophoresis 20180208 7

Two-dimensional gel electrophoresis (2DE) in proteomics is traditionally assumed to contain only one or two proteins in each 2DE spot. However, 2DE resolution is being complemented by the rapid development of high sensitivity mass spectrometers. Here we compared MALDI-MS, LC-Q-TOF MS and LC-Orbitrap Velos MS for the identification of proteins within one spot. With LC-Orbitrap Velos MS each Coomassie Blue-stained 2DE spot contained an average of at least 42 and 63 proteins/spot in an analysis of ...[more]

PMID: 29205401

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