Proteomics

Dataset Information

87

The EML4-ALK Interactome in Lung Cancer


ABSTRACT: We experimentally generated an ALK interactome combining quantitative tyrosine phosphoproteomics and protein complex annotation using affinity purification coupled with mass spectrometry and further investigated downstream ALK signaling using quantitative tyrosine phosphoproteomics.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Homo sapiens  

TISSUE(S): Lung

DISEASE(S): Lung Cancer

SUBMITTER: John Koomen  

LAB HEAD: Eric B. Haura, MD

PROVIDER: PXD004935 | Pride | 2018-10-26

REPOSITORIES: Pride

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Publications

Coupling an EML4-ALK-centric interactome with RNA interference identifies sensitizers to ALK inhibitors.

Zhang Guolin G   Scarborough Hannah H   Kim Jihye J   Rozhok Andrii I AI   Chen Yian Ann YA   Zhang Xiaohui X   Song Lanxi L   Bai Yun Y   Fang Bin B   Liu Richard Z RZ   Koomen John J   Tan Aik Choon AC   Degregori James J   Haura Eric B EB  

Science signaling 20161018 450


Patients with lung cancers harboring anaplastic lymphoma kinase (ALK) gene fusions benefit from treatment with ALK inhibitors, but acquired resistance inevitably arises. A better understanding of proximal ALK signaling mechanisms may identify sensitizers to ALK inhibitors that disrupt the balance between prosurvival and proapoptotic effector signals. Using affinity purification coupled with mass spectrometry in an ALK fusion lung cancer cell line (H3122), we generated an ALK signaling network an  ...[more]

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