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Complementary metaproteomic approaches to assess marine bacteriopankton response towards algal bloom

ABSTRACT: In this project, the metaproteome of the marine bacterioplankton was analyzed to assess its respone towards an algal bloom in the southern North Sea in spring 2010. Proteins were extracted applying two different methods: (i) applying chemical cell lysis using trifluoroethanol in combination with in-solution digest and (ii) mechanical cell lysis applying bead beating, SDS-PAGE prefractionation and in-gel digest. Both samples were analyzed by nanoLC and ESI-iontrap MS. In case of the TFE lysis samples, also nanoLC-MALDI-TOF MS was applied.

INSTRUMENT(S): amaZon Speed ETD

ORGANISM(S): Environmental Samples

SUBMITTER: Lars Wöhlbrand

LAB HEAD: Lars Wöhlbrand

PROVIDER: PXD004944 | Pride | 2017-04-11

REPOSITORIES: Pride

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Complementary Metaproteomic Approaches to Assess the Bacterioplankton Response toward a Phytoplankton Spring Bloom in the Southern North Sea.

Wöhlbrand Lars L Wemheuer Bernd B Feenders Christoph C Ruppersberg Hanna S HS Hinrichs Christina C Blasius Bernd B Daniel Rolf R Rabus Ralf R

Frontiers in microbiology 20170324

Annually recurring phytoplankton spring blooms are characteristic of temperate coastal shelf seas. During these blooms, environmental conditions, including nutrient availability, differ considerably from non-bloom conditions, affecting the entire ecosystem including the bacterioplankton. Accordingly, the emerging ecological niches during bloom transition are occupied by different bacterial populations, with <i>Roseobacter</i> RCA cluster and SAR92 clade members exhibiting high metabolic activity ...[more]

PMID: 28392779

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