AP-MS analysis of m7GTP-binding proteins in Murine norovirus infected BV-2 cells (v2)

ABSTRACT: SILAC-labelling analysis of murine BV-2 cells infected at high multiplicity of infection (10 TCID50/cell) and harvested at 4h or 9h post-infection. Lysates were then suject to m7GTP-sepharose affinity purification to enrich for translation initiation factors. As norovirus translation uses a VPg protein covalently linked to the 5' of its RNAs for initiation factor recruitment it was hypothesised that norovirus infection could modify initiation factor complexes. A paired dataset investigates the effects of MNV infection on total protein levels in infected cells. This dataset is a Maxquant reanalysis of data previously submitted under PXD004019

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Dendritic Cell, Cell Culture

SUBMITTER: Edward Emmott  

LAB HEAD: Ian Goodfellow

PROVIDER: PXD004983 | Pride | 2017-01-17

REPOSITORIES: Pride