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AP-MS analysis of m7GTP-binding proteins in Murine norovirus infected BV-2 cells (v2)

ABSTRACT: SILAC-labelling analysis of murine BV-2 cells infected at high multiplicity of infection (10 TCID50/cell) and harvested at 4h or 9h post-infection. Lysates were then suject to m7GTP-sepharose affinity purification to enrich for translation initiation factors. As norovirus translation uses a VPg protein covalently linked to the 5' of its RNAs for initiation factor recruitment it was hypothesised that norovirus infection could modify initiation factor complexes. A paired dataset investigates the effects of MNV infection on total protein levels in infected cells. This dataset is a Maxquant reanalysis of data previously submitted under PXD004019

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Dendritic Cell, Cell Culture

SUBMITTER: Edward Emmott

LAB HEAD: Ian Goodfellow

PROVIDER: PXD004983 | Pride | 2017-01-17

REPOSITORIES: Pride

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Norovirus-Mediated Modification of the Translational Landscape via Virus and Host-Induced Cleavage of Translation Initiation Factors.

Emmott Edward E Sorgeloos Frederic F Caddy Sarah L SL Vashist Surender S Sosnovtsev Stanislav S Lloyd Richard R Heesom Kate K Locker Nicolas N Goodfellow Ian I

Molecular & cellular proteomics : MCP 20170113 4 suppl 1

Noroviruses produce viral RNAs lacking a 5' cap structure and instead use a virus-encoded viral protein genome-linked (VPg) protein covalently linked to viral RNA to interact with translation initiation factors and drive viral protein synthesis. Norovirus infection results in the induction of the innate response leading to interferon stimulated gene (ISG) transcription. However, the translation of the induced ISG mRNAs is suppressed. A SILAC-based mass spectrometry approach was employed to analy ...[more]

PMID: 28087593

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AP-MS analysis of m7GTP-binding proteins in Murine norovirus infected BV-2 cells

Project description:SILAC-labelling analysis of murine BV-2 cells infected at high multiplicity of infection (10 TCID50/cell) and harvested at 4h or 9h post-infection. Lysates were then suject to m7GTP-sepharose affinity purification to enrich for translation initiation factors. As norovirus translation uses a VPg protein covalently linked to the 5' of its RNAs for initiation factor recruitment it was hypothesised that norovirus infection could modify initiation factor complexes. A paired dataset investigates the effects of MNV infection on total protein levels in infected cells (PXD004015).

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Project description:SILAC-labelling analysis of murine BV-2 cells infected at high multiplicity of infection (10 TCID50/cell) and harvested at 4h or 9h post-infection. Represents the first quantitative proteomics analysis of norovirus-infected cells. A paired AP-MS dataset investigates the effects of MNV infection on eukaryotic initiation factor (eIF) complex formation with this dataset providing data on the overall abundance of eIF components in infected cells.

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