Ontology highlight
ABSTRACT:
INSTRUMENT(S): LTQ Orbitrap
ORGANISM(S): Equus Caballus (horse)
TISSUE(S): Cell Culture
SUBMITTER: Ulla Rüetschi
LAB HEAD: Ulla Ruetschi
PROVIDER: PXD004993 | Pride | 2018-01-05
REPOSITORIES: Pride
Svala Emilia E Löfgren Maria M Sihlbom Carina C Rüetschi Ulla U Lindahl Anders A Ekman Stina S Skiöldebrand Eva E
Connective tissue research 20150422 4
The molecular aspects of inflammation were investigated in equine articular cartilage explants using quantitative proteomics. Articular cartilage explants were stimulated with interleukin (IL)-1β in vitro for 25 days, and proteins released into cell culture media were chemically labeled with isobaric mass tags and analyzed by liquid chromatography-tandem mass spectrometry. A total of 127 proteins were identified and quantified in media from explants. IL-1β-stimulation resulted in an abundance of ...[more]