Label-free Proteomics Reveals that Cowpea Severe Mosaic Virus Transiently Suppresses the Host Leaf Protein Accumulation During the Compatible Interaction with Cowpea (Vigna unguiculata [L.] Walp.)

ABSTRACT: Viruses are important plant pathogens that threaten diverse crops worldwide. Diseases caused by Cowpea severe mosaic virus (CPSMV) have drawn attention because of the serious damages they cause to economically important crops including cowpea. This work was undertaken to quantify and identify the responsive proteins of a susceptible cowpea genotype infected with CPSMV, in comparison with mock-inoculated controls, using label-free quantitative proteomics and databanks, aiming at providing insights on the molecular basis of this compatible interaction. Cowpea leaves were mock-inoculated or inoculated with CPSMV and 2 and 6 days later proteins were extracted and analyzed. More than 3000 proteins were identified and 75 and 55 of them differentially accumulated in response to CPSMV, at 2 and 6 DAI, respectively. At 2 DAI, 76% of the proteins were down-represented and 24% upaccumulated. However, at 6 DAI, 100% of the identified proteins were up-accumulated. Thus CPSMV transiently suppresses the synthesis of proteins involved particularly in the redox homeostasis, protein synthesis, defense, stress, RNA/DNA metabolism, signaling, and other functions, allowing viral invasion and spread in cowpea tissues. It is expected that identification of differentially accumulated proteins and their interactions advance our understanding on how a susceptible cowpea genotype responds to CPSMV infection.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Vigna Mungo (rice Bean) (black Gram)

SUBMITTER: Gustavo De Souza  

LAB HEAD: Luiza Paiva

PROVIDER: PXD005025 | Pride | 2016-12-14

REPOSITORIES: Pride