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QuaNCAT analysis comparing proteins synthesized in neurites and soma in ASCL1-iNeurons

ABSTRACT: Local translation in ASCL1-iNeurons was probed using QuaNCAT

INSTRUMENT(S): Q Exactive

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Embryonic Stem Cell

SUBMITTER: Erik McShane

LAB HEAD: Marina Chekulaeva

PROVIDER: PXD005059 | Pride | 2018-10-17

REPOSITORIES: Pride

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RNA localization is a key determinant of neurite-enriched proteome.

Zappulo Alessandra A van den Bruck David D Ciolli Mattioli Camilla C Franke Vedran V Imami Koshi K McShane Erik E Moreno-Estelles Mireia M Calviello Lorenzo L Filipchyk Andrei A Peguero-Sanchez Esteban E Müller Thomas T Woehler Andrew A Birchmeier Carmen C Merino Enrique E Rajewsky Nikolaus N Ohler Uwe U Mazzoni Esteban O EO Selbach Matthias M Akalin Altuna A Chekulaeva Marina M

Nature communications 20170919 1

Protein subcellular localization is fundamental to the establishment of the body axis, cell migration, synaptic plasticity, and a vast range of other biological processes. Protein localization occurs through three mechanisms: protein transport, mRNA localization, and local translation. However, the relative contribution of each process to neuronal polarity remains unknown. Using neurons differentiated from mouse embryonic stem cells, we analyze protein and RNA expression and translation rates in ...[more]

PMID: 28928394

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