Proteomic analysis of Kveim reagent identifies targets of cellular immunity in sarcoidosis

ABSTRACT: Kveim-reagent (Kv) skin testing was a historical method of diagnosing sarcoidosis. Intradermal injection of treated sarcoidosis spleen tissue resulted in a granuloma response at injection site by 4-6 weeks. Previous work indicates proteins as the possible trigger of this reaction. We aimed to identify Kv-specific proteins and characterise the ex vivo response of Peripheral Blood Mononuclear Cells (PBMCs) from sarcoidosis, tuberculosis and healthy control patients when stimulated with both Kv and selected Kv-specific proteins.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Spleen

SUBMITTER: Robert Parker  

LAB HEAD: Ajit Lalvani

PROVIDER: PXD005150 | Pride | 2017-01-31

REPOSITORIES: Pride